|
|
|
Registros recuperados: 122 | |
|
|
Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul. |
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins... |
Tipo: Text |
Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf |
| |
|
| |
|
| |
|
|
Cordier, Clémence; Guyomard, Killian; Stavrakakis, Christophe; Sauvade, Patrick; Coelho, Franz; Moulin, Philippe. |
The culture of microalgae is important for the production and maintenance of bivalves. One of the major challenges is to maintain the reliability of microalgae forages over the long term. The aim of this work is to use Ultrafiltered (UF) seawater to cultivate them. Thus, cultures in a volume of 300 L of 2 species of microalgae Tetraselmis and T-isochrysis, were monitored in UF water (membrane pore size: 20 nm) and in sea water usually used on the Ifremer mollusk experimental platform of Bouin (France) (Prefiltration, 3 filtrations and 2 UV). The major result is the securing of microlagae cultures with the absence of parasites in all cultures supplied with ultrafiltered water, unlike analyses of the various control cultures. In the case of T-isochrysis, 3... |
Tipo: Text |
Palavras-chave: Microalgae; Ultrafiltration; Water Purification; Shellfish Production. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74182/73785.pdf |
| |
|
|
Dupraz, Valentin; Stachowski-haberkorn, Sabine; Wicquart, Jérémy; Tapie, Nathalie; Budzinski, Hélène; Akcha, Farida. |
Pesticides used in viticulture create a potential risk for the aquatic environment due to drift during application, runoff and soil leaching. The toxicity of sixteen pesticides and one metabolite were evaluated on the growth of two marine microalgae, Tisochrysis lutea and Skeletonema marinoi, in 96-h exposure assays conducted in microplates. For each substance, concentrations of stock solutions were analytically measured and abiotic assays were performed to evaluate the chemical stability of pesticides in microplates. For two chemicals, microalgae exposures were run simultaneously in microplates and culture flasks to compare EC50 calculated from the two exposure systems. Results from chemical analyses demonstrated the low stability of hydrophobic... |
Tipo: Text |
Palavras-chave: Microalgae; Microplates; Screening; Pesticides; Toxicity; Exposure characterisation. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00474/58558/61113.pdf |
| |
|
|
Nassif, L. Abi; Rioual, S.; Trepos, Rozenn; Fauchon, M.; Farah, W.; Hellio, C.; Abboud, M.; Lescop, B.. |
Microorganisms have the ability to settle on nearly all man-made surfaces in contact with seawater and subsequently to form biofilm. Biofilms control and removal is necessary in the sectors of maritime transport, energy... In this work, we present the development of new pure calcium, zinc or copper alginate, but also mixed Ca/Cu and Ca/Zn alginate hydrogels. These materials have been evaluated for their potential inhibition of adhesion of two key biofilm-forming microalgae Halamphora coffeaeformis and Cylindrotheca closterium). All the tested materials have presented high adhesion inhibition about 80%). Copper-base materials present a high toxicity against H. coffeaeformis. Pure zinc alginate is also toxic for this strain. However, the addition of calcium... |
Tipo: Text |
Palavras-chave: Biofilm; Adhesion; Alginate; Zinc; Biomaterials; Microalgae. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00605/71671/70168.pdf |
| |
|
| |
|
|
Bartozek,Elaine Cristina Rodrigues; Bueno,Norma Catarina; Ludwig,Thelma Alvim Veiga; Tremarin,Priscila Izabel; Nardelli,Margaret Seghetto; Rocha,Angélica Cristina Righetti da. |
This study represents a taxonomic survey of class Bacillariophyceae diatoms found in samples collected in the Iguaçu River (Iguaçu National Park, municipality of Foz do Iguaçu, in the state of Paraná, Brazil) between August 2007 and July 2008. Two sampling stations were utilized, the first located upstream of Iguaçu Falls (Cais Bananeiras Station) and second downstream of the falls (Cais Macuco Safari Station). The study resulted in the identification of 86 taxa, including 74 at the specific level and seven taxonomic varieties. Two of these had never before been documented in the state of Paraná: Gomphonema gibberum Hustedt and Pinnularia meridiana Metzeltin & Krammer. Nine are first-time records for Brazil: Cymbella charrua Metzeltin,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Taxonomy; Microalgae; Lotic system; State of Paraná. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000100012 |
| |
|
|
Teixeira,Priscila F.; Kaminski,Sonia M.; Avila,Tatiana R.; Cardozo,Alessandro P.; Bersano,José G.F.; Bianchini,Adalto. |
Egg production in the copepod Acartia tonsa was evaluated using different densities of the microalgae Thalassiosira weissflogii, Chaetoceros muelleri and Isochrysis galbana. Male and female were kept under controlled conditions (salinity 30, 20°C, photoperiod 12L:12D), acclimated to the experimental conditions and left over a period of 24 h to allow copulation. Algal densities tested were equivalent in biovolume and corresponded to 0, 2.5, 5, 10, 20, 40 and 60.10³ cells.mL-1 of T weissflogii. Ten acclimated female were separated, transferred to glass bottles and exposed for further 24 h to the corresponding experimental medium. After this period, the eggs were fixed and counted. Copepod egg production reached a threshold value when T weissflogii, C.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg production; Acartia tonsa; Microalgae. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200009 |
| |
|
|
Rossa-Feres,Denise de C.; Jim,Jorge; Fonseca,Mariluce Gonçalves. |
The diet of tadpoles of 13 anuran species was determined to verify whether food resource partitioning occurs and whether the degree of diet similarity is related to taxonomic affinity. Tadpoles of all species studied were mainly herbivorous, except for these of Leptodactylus fuscus (Schneider, 1799) which were mycophagous. Although some species had exclusive items in their diet, most tadpole species ingested the same items, but differed in the amount of each item consumed. Two guilds were found: tadpoles that feed on diatoms on the pond bottom, and tadpoles that feed on Oedogonium Link, 1820 algae in midwater. Diet similarity was related to the taxonomic relationship, microhabitat and feeding behavior of tadpoles indicating that the community organization... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Community ecology; Food partitioning; Microalgae. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400003 |
| |
|
| |
|
|
Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert. |
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes. |
Tipo: Text |
Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf |
| |
|
| |
|
|
Cognie, B; Barille, Laurent. |
Bivalves, by releasing inorganic nutrients, can stimulate the growth of the primary producers on which they feed. Dissolved excretion and mineralization of biodeposits (pseudofaeces and faeces), two indirect processes related to their feeding activity, have already been identified. This study investigated whether microalgal growth is also dependent on the mucus secreted by bivalves and rejected with their biodeposits. An experimental study based on algal growth tests was conducted to determine whether the mucus coating the pseudofaeces and faeces of the oyster Crassostrea gigas (Thunberg) could stimulate the growth of nine marine microalgae: a Prasinophyceae [Tetraselmis suecica (Butcher)] and eight Diatomophyceae [Amphora sp. (Ehrenberg), Chaetoceros sp.... |
Tipo: Text |
Palavras-chave: Bivalve; Microphyte; Mucus; Fèces; Pseudofèces; Bivalve; Microalgae; Mucus; Faeces; Pseudofaeces. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00325/43607/43926.pdf |
| |
|
|
Carrier, Gregory; Baroukh, Caroline; Rouxel, Catherine; Duboscq-bidot, Laetitia; Schreiber, Nathalie; Bougaran, Gael. |
Tisochrysis lutea is a microalga species currently used in aquaculture as a feed for shellfish, oysters and shrimps. It also has many other potential industrial applications, such as the production of neutral lipids for biofuels or the production of ω-3 fatty acids for nutraceuticals (human food complements). To efficiently exploit the potential of this microalga, however, higher lipid productivities are needed. To this end, improvement programs need to be developed and optimized. The diversity of strains available in microalgae has not yet been exploited in such improvement programs. In this study, the intra-strain diversity was observed and exploited to increase neutral lipid productivity. New clonal strains with higher neutral lipid productivity were... |
Tipo: Text |
Palavras-chave: Microalgae; Genome; Improvement program; Lipids; Tisochrysis lutea. |
Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00411/52204/52927.pdf |
| |
|
|
Martínez, Carlos; Mairet, Francis; Martinon, Pierre; Bernard, Olivier. |
Microalgae cultivation with wastewater is a promising way of reducing the energetic needs for wastewater treatment and the costs of biofuel production. However, the very turbid medium is not favorable for the development of microalgae. Indeed, light, the key element for photosynthesis, rapidly vanishes along depth due to absorption and scattering. Therefore it is crucial to understand the effects of the depth on turbid cultures. In this work, we study theoretically the long-term behavior of a continuous culture of microalgae exposed to a periodic source of light. By allowing periodic variations of the depth and the hydraulic retention time, we show that the microalgae population is forced to a periodic regime. Finally, we address numerically the problem of... |
Tipo: Text |
Palavras-chave: Dynamics and control; Industrial biotechnology; Wastewater; Biomass productivity; Microalgae. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61623/65654.pdf |
| |
|
|
Martinez, Carlos; Mairet, Francis; Bernard, Olivier. |
The periodically forced light-limited Droop model represents microalgae growth under co-limitation by light and a single substrate, accounting for periodic fluctuations of factors such as light and temperature. In this paper, we describe the global dynamics of this model, considering general monotone growth and uptake rate functions. Our main result gives necessary and sufficient conditions for the existence of a positive periodic solution (i.e. a periodic solution characterized by the presence of microalgae) which is globally attractive. In our approach, we reduce the model to a cooperative planar periodic system. Using results on periodic Kolmogorov equations and on monotone sub-homogeneous dynamical systems, we describe the global dynamics of the... |
Tipo: Text |
Palavras-chave: Variable quota model; Positive periodic solution; Global stability; Microalgae; Cooperative system. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00599/71140/69455.pdf |
| |
|
| |
|
|
Jauffrais, Thierry; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp. |
Azadinium spinosum, a small dinoflagellate has recently been discovered and identified as the primary producer of azaspiracid-1 (AZA) and -2. Since AZA poisoning has been reported following consumption of contaminated shellfish it is important to have these toxins available for toxicological studies, and a sustainable production of AZAs as calibrants in monitoring programs without having to rely on natural events. In order to address this concern, continuous pilot scale cultures were carried out to evaluate the feasibility of AZA production from A. spinosum. Algae were cultured using two 100 L chemostats in series (R1 and R2), with agitation and pH control. Four different dilution rates were tested (0.15, 0.2, 0.25 and 0.3 day−1) to evaluate chemostat... |
Tipo: Text |
Palavras-chave: Solid phase adsorption; Chemostat; Photobioreactor; Microalgae; LC-MS/MS; Tangential flow filtration. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00152/26349/24437.pdf |
| |
|
| |
Registros recuperados: 122 | |
|
|
|